Volume 2, Issue 4, August 2017, Page: 31-35
Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)
Ayuba Yohanna Musa, Department of Chemistry, Abubakar Tafawa Balewa University Bauchi, Bauchi, Nigeria
Okoro Benedict Chidiogor, Department of Microbiology, Godfrey Okoye University Emene, Enugu, Nigeria
Abubakar Muhammad Nazif, Department of BioTechnology, Modibbo Adama University of Technology Yola, Adamawa, Nigeria
Ilesanmi Esther, Department of Chemistry, University of Benin, Benin, Nigeria
Received: Aug. 22, 2017;       Accepted: Sep. 5, 2017;       Published: Sep. 30, 2017
DOI: 10.11648/j.ijbbmb.20170204.12      View  1403      Downloads  106
Abstract
Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.
Keywords
Phytochemical, Antimicrobial, TLC, Staphylococcus Aureus, Candida Albicans, Bacillus Subtillis
To cite this article
Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther, Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L), International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 2, No. 4, 2017, pp. 31-35. doi: 10.11648/j.ijbbmb.20170204.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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